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Berkala Penelitian Hayati

jurnal
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Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur

ISSN : 08526834 EISSN : 2337389X DOI : https://doi.org/10.23869/bphjbr

Core Subject : Health, Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 10 Documents

Search results for , issue "Vol 21 No 1 (2015): December 2015" : 10 Documents clear

Speciation and zoogeography of amphibian in Sundaland Nia Kurniawan; Driyana Rike Ahmadlia; Day Shine Nahari; Anggun Sausan Firdaus
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 21 No 1 (2015): December 2015
Publisher : The East Java Biological Society

Sundaland is an interesting area to be explored based on its geological history, topography, and climate. Sundaland consists of Penisular Malaysia, Sumatra, Borneo, and Java which experienced some emergence and submergence process in the past. During 1981-2015, most of research in Sundaland found that amphibian family in Sundaland was dominated by Bufonidae, Ranidae, Microhylidae, Megophrydae, Rachophoridae, and Dicroglossidae which experienced lot of speciation in its history. Among of 4 major islands in Sundaland, Borneo has the highest number of species diversity, then Peninsular Malaysia, Sumatra, and Java. During those years, Sumatra and Java got least concern by researcher. Therefore, it is suggested for further study to explore more in Sumatra and Java.

The effect of root and litter on soil macroporosity of Terminalia microcarpa and Dillenia philippinensis in Purwodadi Botanical Garden Agung Sri Darmayanti; Esti Endah Ariyanti; Luchman Hakim
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 21 No 1 (2015): December 2015
Publisher : The East Java Biological Society

D. philippinensis or Sempur (Java) and T. microcarpa are dominated most of vegetation in Purwodadi Botanical Garden area that will be muddy after rain. This condition can be suspected as the effect of low soil macroporosity. This study aim was to observe the effect of root quantity and litter quality surround Terminalia and Dillenia trees. Root quantity parameters which observed were Lrv (Root Length Density) dan Drv (Dry Root Mass). Sample was taken using drilling method and roots were measured using line interception method. Soil macroporosity was measured using methylene blue staining. Litter decomposed in forest floor, it weight then measures on 12 weeks after taken. Soil macroporosity higher in soil surround Dillenia than Terminalia. Soil macroporosity surround Terminalia and Dillenia are not signifi[1]cantly correlated to Lrv (Root Length Density) dan Drv (Dry Root Mass). While, decomposition rate of litter has corre[1]lated to macroporosity, especially on Terminalia.

Microbial diversity and its importance in microbial genetic resources preservation and its role in natural Langkah Sembiring
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 21 No 1 (2015): December 2015
Publisher : The East Java Biological Society

Biodiversity has been very common to almost every one. However, a comprehensive understanding on the concept of biodiversity might be less common to many discussing parties. Likewise, microbial diversity is even less common to most of academician, ex-cept of course to microbiologists. In fact, a correct and clear concept of biodiversity is prerequisite for serious and appropriate discussion of the matter. In this paper, the concept and understanding of microbial diversity is fundamentally described as well as their genetic potential by reviewing the development and application of species concept based on molecular biological approach. It is an undeniable fact that molecular biology has provided a powerful tool for microbiologists as well as evolutionists to unravel the biodiversity of microbial world which play a paramount important to conserve the basic function of any natural environments in the biosphere since microbes live and flourish in all ecosystems, including extreme habitats. The ubiquity of microbes clearly underpins by their diversity, including physiological and metabolic diversity, ability to live in anaerobic environments, and their small size. Molecular biology development and application in microbiology have transformed the three areas in microbiology, namely microbial ecology, microbial diversity, and microbial evolution from weakness into the strength, in unraveling and understanding microbial diversity and its genetic potential as well as its role in nature, especially their role to keep work the biogeochemical cycle in the Earth. Only by having an adequate understanding of microbial critical role in preserving nature that the environmental conservation issue could be carried out, understood and realized meaningfully.

Characterization of protease-producing bacteria isolated from terasi Novi Arfarita; Ekachai Chukeatirote
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 21 No 1 (2015): December 2015
Publisher : The East Java Biological Society

Total of 117 bacterial strains were isolated from terasi samples and 69% of isolates (71) could perform distinctive proteolytic activity that related to the ability to produce protease enzymes. Their proteolytic activity was further tested using spot incubation technique. Strain S4-5 has shown the highest activity then was selected for further tests in this study. Gram staining test showed that S4-5 is gram positive bacteria and able to grow under an aerobic condition. Based on API biochemical profiles, S4-5 strain bacteria was Bacillus licheniformis. Similarity test of genome sequence among Bacillusspecies from gene bank (EMBL Sequence Version) with Bacillusspp., strain S4-5 had similarity with Bacillus licheniformis genome. The optimal pH of this strain was 6 whereas the optimum temperature for Bacillus licheniformis strain S4-5 was 37°C.

Distribution and characteristic of deposit-feeder crabs (Crustacea: Brachyura) in some mangrove ecosystem types in Lombok Island Dewi Citra Murniati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 21 No 1 (2015): December 2015
Publisher : The East Java Biological Society

Lombok Island consists of five different mangrove forest types with various pattern of fauna distribution. These pattern variations were constructed by different type of substrates. Deposit feeder crabs play an important role in determination of substrate pattern. The objective of this study was to investigate the distribution pattern of some crabs which their characteristics are correlated to the mangroves substrate. Crabs were collected from eight locations in Mangrove area of Lombok Island. Ten species of the deposit feeder crab were identified, their distribution pattern was illustrated based on the result of field observation on their morphological characteristics. The highest species diversity found in Teluk Rasu and Tanjung Luar. Both cheliped and maxilliped characteristics represent to habitat preference. Uca annulipes, U. perplexa, U. vocans, and Dotilla myctiroides inhabited sandy substrate, while U. bellator, U. demani, U. dussumieri, U. forcipata, U. triangularis, and Tmethypocoelis ceratophora inhabit muddy substrate.

The expression of TLR-2 and NOD-2 in gingival epithelium of rat after probiotic Lactobacillus reuteri supplementation to inhibit Streptococcus mutans growth Tuti Kusumaningsih; Subijanto Marto Sudarmo; Retno Indrawati; Rini Devijanti Ridwan; Sidarningsih Sidarningsih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 21 No 1 (2015): December 2015
Publisher : The East Java Biological Society

Lactobacillus reuteri is probiotic from Gram positive bacteria which has specific molecular structure, consisted of peptidoglycan (PG) and lipotheihoic acid (LTA). These structure have potential in pattern recognition receptors (PRRs) activation, such as TLR-2 and NOD-2 that are the up stream of beta defensin-2 (BD-2) signaling cascade. BD-2 is antimocrobial peptides naturally produced in mouth cavity that can against Streptococcus mutans effectively. This study was aimed to prove that probiotic L. reuteri supplementation can increase the expression of TLR-2 and NOD-2 in gingival epithelium. Experimental design in this study was randomized control group post test only design. Study was carried on 24 white rat (Rattus norvegicus) Wistar strain which divided into 4 groups. Positive control was rats that induced with S. mutans, while rats in negative control group were not induced. Group I was rats that suppelemented with L. reuteri for 14 days (day 1-14) and induced with S. mutans for 7 days (day 8-14). Group II was rats that supplemented with L. reuteri and induced with S. mutans simustaneously for 14 days (day 1-14). Concentration of bacterial suspension was 108 cfu ∕ml for L. reuteri and 1010 cfu/ml for S. mutans. Both of these two bacteria was given orally to rats. TLR-2 and NOD-2 expressions were evaluated with immunohistochemistry technique. Significant differences of protein expression between each treatment groud was analyzed with ANOVA (p=0.001). TLR-2 and NOD-2 expressions were higher than negative control. It can be conclude that L. reuteri supplementation as probiotic could increase the expression of TLR-2 and NOD-2 in gingival epithelium of rat.

ANTIBACTERIAL ACTIVITY OF BELILIK (Brucea javanica (L). MERR) AND BENTA (Wikstroemia androsaemofolia DECNE) TO INHIBIT THE GROWTH OF ENTEROPATHOGENIC BACTERIA Henny Helmi; Idha Susanti; Noptian Asmara Agung; Sadam Kusen
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 21 No 1 (2015): December 2015
Publisher : The East Java Biological Society

Several native Indonesia plants have been used to prepare traditional medicine since long time ago. One of common diseases in tropical country is diarrhea, it caused by the infection of enteropathogenic bacteria such as Enteropathogenic Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Shigella sp. Belilik (Brucea javanica (L.) Merr) and Benta (Wikstroemia androsaemofolia Decne) are herbals that utilized as medicine for diarrhea in Bangka Belitung, Indonesia. Parts of these plants are mostly can be utilized as medicine, such as leaf, root, and fruit. The aims of this study were to investigate the antibacterial activity of ethanol crude extract of B. javanica (root and fruit) and W. androsaemofolia (leaf and fruit) against enteropathogenic bacteria (EPEC, P. aeruginoa, S. aureus, Shigella sp.). Meth od that used was papper disc diffusion. The results showed that at concentration 10 mg/mL, 20 mg/mL, and 30 mg/mL of B. javanica ethanol extract of both root and fruit could not inhibit the enteropathogenic bacteria, while the ethanol extract of leaf and fruit of W. androsaemofolia were shown inhibition activity on the growth of enteropathogenic bacte ria. W. androsaemofolia leaf extract performed the best inhibition activity to the growth of EPEC (20.55±1.5mm) and S. aureus (22.14±4.5mm), it was better than kanamycin performance at the same concentration (30 mg/mL). In addition, ethanol extract of W. androsaemofolia fruit showed the best inhibition activity against Shigella sp. (19.64±1.8mm).

MORPHOMETRIC CHARACTERS OF MARINE LOCAL FISH (Harpodon sp.) FROM TARAKAN, NORTHERN BORNEO Endik Deni Nugroho; Dwi Anggorowati Rahayu; Moh. Amin; Umie Lestari
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 21 No 1 (2015): December 2015
Publisher : The East Java Biological Society

Tarakan sea waters have geographical circumstances and landscapes that supported the existence richness of fishes that abundant and diverse. There is local fish called Pepija or Lembe-Lembe, but biological studies on this fish most poorly known. This research is aimed to identify the morphological characters of Nomei (Harpodon sp.). Based on specific caharcter, morphometric, and meristric characters. Morphometrics analysis can be used to clustering and understanding relationship of taxa. Each species represented 10% samples of male and female from each type we found and measured 35 characters (in mm). The canonical, clustering and similarity were used. Based on determinant morphological characters species (pectoral fin length reaches or exceeds the initial dorsal fin, dorsal fin earlier than ventral fins), dendogram morphological characters, meristic characters, PCA grouping proved that Nomei fish from Amal and Juata as Harpo-don nehereus.

SCREENING OF RAPD PRIMER FOR TEAK (Tectona grandis) Imas Cintamulya
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 21 No 1 (2015): December 2015
Publisher : The East Java Biological Society

Identification of DNA polymorphisms in teak is important. It is a first step to determine the presence of genetic varia-tion in teak. The information of genetic variation is needed for teak breeding development. RAPD is one of method which can be used for identification of DNA polymorphism. This study aim to get the RAPD primer which can detect the DNA polymorphism in teak. Benefits of this study are provide information about primer which can detect the DNA polymorphism in teak, DNA polymorphism data can be used for genetic variation analysis which needed for teak breeding development. The primers which used in this study shown the DNA polymorphism in teak. The primer are OPF6 (5'-GGGAATTCGG-3 '), OPF8 (5'-GGGATATCGC-3'), and OPF11 (5'-TTGGTACCCC-3 '). The highest DNA polymorphism is shown in DNA which amplified with OPF-8 primer.

THE EXPLORATION OF PLANT SPECIES IN NATURE RESERVE OF MOUNT MUTIS EAST NUSA TENGGARA PROVINCE Solikin Solikin
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 21 No 1 (2015): December 2015
Publisher : The East Java Biological Society

This research was aimed to explore and inventory the plant diversity, especially medicinal plants in Nature Reserve of Mount Mutis. Data were collected in Fatumnasi Village, Fatumnasi District of South Central Timor Regency in October 2011 through plant exploration and interview the local people. Plants inventory was conducted along the tracks during exploration. Herbs vegetation analysis was conducted among the tree stands of Eucalyptus urophylla. In addition, orchid vegetation analysis was only conducted to orchids that have been found attaching to Eucalyptus urophylla trees. Results showed that there were about 52 family, 78 genera and 84 species of plants in the observed area. Tree species was dominated by 'ampupu' (Eucalyptus urophylla), while orchid species was dominated by Eria retusa. Herbaceous plant communities were dominated by Centella asiatica, Cyperus sp. and Cynodon dactylon. There were about eight plant species of medicinal plants and one food plant species found in the forestthat have been known by local people.

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Contact Name
Sutiman Bambang Sumitro

Contact Email
berkalahayati@yahoo.com

Phone
+62341570631

Journal Mail Official
wulidanisa@berkalahayati.org

Editorial Address
Jalan Surakarta No. 5 Malang, Indonesia

Location
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Unknown

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Sutiman Bambang Sumitro

Contact Email berkalahayati@yahoo.com

Phone +62341570631

Journal Mail Official wulidanisa@berkalahayati.org

Editorial Address Jalan Surakarta No. 5 Malang, Indonesia

Location Unknown, Unknown INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Sutiman Bambang Sumitro

Contact Email
berkalahayati@yahoo.com

Phone
+62341570631

Journal Mail Official
wulidanisa@berkalahayati.org

Editorial Address
Jalan Surakarta No. 5 Malang, Indonesia

Location
Unknown,

Unknown

INDONESIA